Bio Patent New Material

Completing Nature’s Wisdom with Technology

Innovative technology unlocking hidden nutrients within seeds, capturing the true value of bokbunja.

Reduction of Triglycerides and LDL Cholesterol – KSCI

Principal Investigator: Hyun-Soo Nam
Ministry of Trade, Industry and Energy Project, 2013

Protecting the Liver from
Oxidative Stress – SCI Level

2017 Mungyeongsan Bokbunja Oil Extraction Macau Medical University, College of Pharmacy, Fujian National Agricultural University Joint paper by Professor Hui Tung

Approval for Phase 1 Clinical Trial by KFDA as a Natural Product-Based New Drug

Conducted at Wonkwang University
Oriental Medicine Hospital
with 30 participants

Conducted at Wonkwang University
Oriental Medicine Hospital
with 30 participants

Antiviral efficacy of patented materials
identified in SCI-class papers

“Influenzaantiviral efficacy” of Rubus Coreanus seed extract

“NorovirusAntiviral Efficacy” by Rubus Coreanus seed

Efficacy of
bio-patented materials

Patent data from Yonsei University: patented
substance reduced prostate cancer cells by 62%

NK cells’ ability to kill target cancer cells increased by 66%.

Spike-ACE2 Binding Neutralization Rate

4 times higher efficacy than squalene in
COVID-19 neutralizing antibody test.

Neutralizing antibody

Antibodies that bind to biologically active substances such as viruses,
toxins, or enzymes to inhibit their pathogenicity or biological activity.

Serum Dilution Ratio	1/50	1/100	1/200	1/400
Saline	0	0	0	0
Spike only	1.2	-2.1	-0.35	2.3
Spike + Alum	31.5	26.9	-8.21	2.4
Spike + Squalene	14.9	11.6	-5.2	4.1
Spike + Rubus Coreanus Oil	61.3	52.7	-37.9	23.4
Spike + Rubus Coreanus Oil +Alum	57.5	36.5	-24.6	10.9

Test results for BaccineAjuvant Antibody & Neutralizing Antibody
by Ewhaon Rubus coreanus seed oil

Vaccine Adjuvant Antibody Test Method

Negative Control	No antigen present.
Antigen Only	Contains only the antigen.
Positive Control 1	Antigen + Alum adjuvant.
Positive Control 2	Antigen + Squalene adjuvant.
Experimental Group 1	Antigen + RC oil.
Experimental Group 2	Antigen + RC oil + Alum adjuvant.

Measurement of Anti-Spike IgG: Sandwich ELISA

ELISA: Tracks changes in antibodies (IgG) in serum
after administering the Spike protein antigen.

Baccine Ajuvant Antibody Test Results

From the Baccine Ajuvant antibody formation rat test shows very good results.
Antibody LgG was formatted after 3 week injection.
And Showed very good results with using RC oil and Alum together.
It means RC oil contribute to formation Antibody.

Neutralizing Antibody MOA

Neutralizing Antibody Test Results

From the test ACE-2 binding and Neutralization,
Shows very good results at all the serum dilution ration.
It means RC oil strongly role blocking
ACE2 binding and Neutralization Luciferase.

Spike-ACE2 Binding Neutralization Rate

Serum Dilution Ratio	1/50	1/100	1/200	1/400
Saline	0	0	0	0
Spike only	1.2	-2.1	-0.35	2.3
Spike + Alum	31.5	26.9	-8.21	2.4
Spike + Squalene	14.9	11.6	-5.2	4.1
Spike + Rubus Coreanus Oil	61.3	52.7	-37.9	23.4
Spike + Rubus Coreanus Oil +Alum	57.5	36.5	-24.6	10.9

Phase 1 results for “Improve Blood Circulation”
by Ewhaon Rubus coreanus seed oil

Summary Phase 1 Test

Test Name	Improve blood circulation by RC seed Oil
Test Organization	WonKwang University KwangJu Hanbang Hospital
Test Aim	Improve blood circulation
Test Product	RC seed oil/Placebo
Applier	J clean berry Co., LTD.
Test period	2014. 07. 22 ~ 2014. 10.17
Subject persons	Total 30(RC seed oil : 15, Placebo : 15)
Test method	2,000mg/cap Oral dose
Test Report	2014. 11. 27
Main Biomarker	PT, aPTT, Fibrinogen, Total chlolesterol, HDL/LDL cholesterol, 5-HT(serotonin) thromboxane B2, TG

Summary Phase 1 Test Results

Total cholesterol and LDL cholesterol were reduced compare with control group.
So RC seed oil have good efficacy for “Improve Blood Circulation”.

→ Approved by KFDA

Alpha linoleic acid concentration as marker compound

Lot No. Times	1(Lot LC08296V)	2(Lot LC08296V)	3(Lot LC08296V)	Average
1	24.80	25.11	25.23	–
2	24.08	25.13	25.26	–
3	24.87	25.03	25.17	–
Average	24.82	25.09	25.22	25.04

Schedule Phase 2 for “Blood Circulation Drug”
by Ewhaon Rubus coreanus seed oil

Summary for Phase 2 Test

Test Name	Blood circulation by RC seed Oil
Test Organization	Have to contact
Test Aim	Blood circulation
Test Product	RC seed oil / Placebo
Applier	EWHAON Co., LTD.
Test period	2014.07.22 ~ 2024.10.17
Subject persons	Total 60(RC seed oil : 30, Placebo : 30)
Test method	2,000mg/cap Oral dose
Test Report	–
Main Biomarker	Total chlolesterol HDL/LDL cholesterol